Easy Detection

The reliability of results is of critical importance when gene amplification is performed for testing and diagnosis. With conventional methods, a separate detection reaction is required after amplification to confirm the gene that has been amplified. This adds to the complexity of genetic testing. On the other hand, the LAMP method accomplishes very specific gene amplification and the results can be judged directly. Furthermore, because it produces amplification products in far larger quantities, a simple method of detection can be selected. For example, if gene amplification is performed in the presence of a fluorescent intercalating dye such as ethidium bromide (EtBr), which binds to double-stranded nucleic acids, amplification products can be detected with the aid of an ultraviolet lamp. The efficiency of amplification is so high that pyrophosphoric acid is formed in large quantities as a product of DNA synthesis. When it combines with magnesium, turbidity is produced. Gene amplification can therefore be confirmed by visual detection of turbidity or a white precipitate.[4] Turbidity can also be measured so that gene amplification can be detected in real-time (Fig. 1B).

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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