Embryo And Polar Body Biopsy

Currently, most centers performing PGD use cells obtained from the six- to eight-cell cleavage stage (day 3 of embryonic development).[1] Embryo biopsy is a two-step procedure in which a hole is first drilled in the zona pellucida surrounding the embryo at this stage, and next, one or two cells are aspirated through it (Fig. 1). The hole made in the zona pellucida is usually 30-50 mm, and it is believed that a larger hole may be detrimental to embryo development and viability.[2] Until recently, most centers used acidified Tyrode's solution for zona drilling, but a noncontact laser method has become very popular and is also widely used for assisted hatching (references in Ref. [1]). No controlled randomized studies have yet been conducted to compare the effects of acid Tyrode's solution or the laser on embryo viability. However, comparative data from the ESHRE PGD Consortium and others have shown no difference in pregnancy rates between these two methods.[1,3] Another controversy is whether

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