Example of Application of RFLP Mycobacterium tuberculosis

The most commonly used typing system for M. tuberculosis is IS6110-based RFLP,[4] so far considered the gold standard for TB typing. IS6110 is an IS3-like element, also known as IS986 or IS987. It occurs at various locations in the genome of M. tuberculosis in variable copy numbers.[5'6] As its sequence is apparently invariant,1-7-1 it is an ideal target sequence. The copy number of the element ranges from 1 to 25 copies per genome, although rare M. tuberculosis cultures have been reported with no inserts. To visualize IS6110 RFLP patterns, DNA is extracted and purified from bacterial culture. Thereafter, the DNA is digested with the restriction enzyme PVuII, the restriction fragments are separated on an agarose gel and transferred to a DNA membrane. A peroxidase-labeled probe with a DNA sequence complementary to the IS6110-DNA sequence is added in order to visualize the IS6110 containing restriction fragments, by chemiluminescence, which is initiated by adding two substrates. Restriction fragment length polymorphism

Slot reference mix, signifying the starting point of electrophoresis

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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