Famccggcccagccaccatggtamra

TCCCAGGTCATCCGTGCTCA-Flu

LCRed705-TTAGCAGCTCATCCAGCTGGGTCAG-Ph

CGACCCCTTACCCGCATCTCCC-Flu

LCRed640-CCCCAAGACGCCCCTTT-Ph

CCTCGGTCACCCACTGCTCCAGC-Flu

LCRed640-CTTCTTGCCCAGGCCCAAGTTGC-Ph

ACATTCGGAGGTAGGATCATGAGCA-Flu

LCRed640-CCCCAGGCCAGCGTGGTCGA-Ph

CCCAGTTCCCGCTTTGTGCCCTTC-Flu

LCRed705-CCCATCACCCACAGGAGTGGTT-Ph

GTGAGGCAGGTATGGGGCTAGAAGCACTG-Flu

LCRed640-GCCCCTGGCCGTGATAGTG-Ph

Table 3 Sequences of the probes used for real-time PCR of CYP1A2

Detected allele Sequence of probe 5'-3' Reference

*1F GGGCCCAGGACGCAT-Flu [20]

LCRed640-GTAGATGGAGCTTAGTCTTTCTGGTATCCA-Ph

Phenotyping using caffeine showed no difference in CYP1A2*1F metabolic activity between different genotypes in nonsmokers but a significant difference in smokers homozygous for the A allele compared with the other genotypes in terms of a 1.6-fold increased activity.1-17-1 Unexpectedly, another study demonstrated a lower in vivo activity in patients with colorectal cancer than in healthy controls.[19]

The frequency of the *1F allele seems to be higher in Caucasians compared with Japanese. The study which investigated this new polymorphism analyzed 159 healthy Japanese and detected a prevalence of 39% homozygous and 44.6% heterozygous subjects.[18] In contrast, another study examined 236 Caucasians and found 46% homozy-gous A/A alleles and 44% heterozygous A/C alleles.[17]

In routine genotyping of CYP1A2, only *1F and a second allele, *1D, the deletion of T at position — 2464, need to be analyzed because all other CYP1A2 polymorphisms are in linkage disequilibrium with these two alleles.[19]

At present, only one study has described real-time PCR methodology for the detection of CYP1A2*1F. This was performed employing the LightCyclerâ„¢ instrument and fluorescent hybridization probes and melting curve analysis.[20] (For detailed information of the probes, see Table 3.)

The Smoker's Sanctuary

The Smoker's Sanctuary

Save Your Lungs And Never Have To Spend A Single Cent Of Ciggies Ever Again. According to a recent report from the U.S. government. Centers for Disease Control and Prevention, more than twenty percent of male and female adults in the U.S. smoke cigarettes, while more than eighty percent of them light up a cigarette daily.

Get My Free Ebook


Post a comment