Filoviruses

Ebola and Marburg viruses can be detected by Filovi-ridae-specific primers binding to the polymerase gene. These primers target sites that are highly conserved among the virus family[4] and were applied in PCRs of conventional and real-time format (Table 1, PCR 1-3). The glycoprotein gene of Ebola virus is used as a target to detect all four subtypes of Ebola virus (Zaire, Sudan, Ivory Coast, and Reston), but not Marburg virus (Table 1, PCR 4).[4] Furthermore, real-time PCRs in the glycopro-tein gene for differentiating Zaire and Sudan strains, as well as for detecting Marburg virus, are available (Table 1, PCR 7 and 8).[7,8] PCR tests targeting the nucleoprotein gene detect and differentiate Ebola subtypes Zaire and Reston (Table 1, PCR 5 and 6). However, differentiation between filovirus species or subtypes is not required in the clinical situation.

Sensitivity studies have been mainly carried out using the polymerase gene-specific PCR. The clinical sensitivity of this PCR was 100% in two studies and thus higher than virus isolation or antigen capture assay.[4,5] Furthermore, even in patients with silent seroconversion, Ebola virus RNA can be detected in PBMC by a nested format of the

Table 1 Filovirus PCR
Swine Influenza

Swine Influenza

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