General Considerations Of Ptt Sensitivity of PTT

The PTT is most often used to detect truncating mutations in tumor suppressor genes from patients that are heterozygous for the mutation, using genomic DNA as starting material. The mutant allele represents 50% of the alleles present in the sample, and the PTT, like all other scanning methodologies, can easily detect mutations at the

50% level. There are other applications, however, where the mutant allele is present at much less than 50% (e.g., in a tumor biopsy or fecal material from a colorectal cancer patients) and in these cases sensitivity becomes an issue. In comparison with other mutation detection techniques, the sensitivity of gel-PTT is high and is capable of detecting mutant alleles at one part in five. Recently, Traverso et al. have demonstrated detection efficiencies of chain truncation mutations as low as 0.4% relative to WT.[15] This was achieved by first diluting genomic DNA samples so that no more than two to four DNA templates are present in each sample prior to PCR amplification. This is followed by translation of the amplified DNA for approximately 144 samples and detection using radioactive gel-based PTT. As discussed previously,1-9-1 ELISA-PTT is ideal for such an application as radioactive gel-based detection is not suitable for automation.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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