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PCR cycles

Fig. 1 Generation of an HTLV-1 proviral DNA standard curve. (A) Amplification plot of a serial dilution of the HTLV-1 pBR322 vector plasmid. (B) HTLV-1 standard curve generated by the Ct values in (A) for a known amount of template molecules. (View this art in color at www.dekker.com.)

PCR cycles

Fig. 1 Generation of an HTLV-1 proviral DNA standard curve. (A) Amplification plot of a serial dilution of the HTLV-1 pBR322 vector plasmid. (B) HTLV-1 standard curve generated by the Ct values in (A) for a known amount of template molecules. (View this art in color at www.dekker.com.)

designs and PCR technology applied. In our laboratory, the PCR reaction has been carried out using the LightCycler Technology assay instrument. For each test sample, 30 ng of genomic DNA is used as a template for amplification using LightCycler-FastStart DNA Master Hybridization Probes Kit (Roche Diagnostics GmbH) in the LightCycler PCR instrument, and then the amplified products are automatically measured according to the manufacturer's instructions. The reaction conditions are 95 °C for 10 min for activation of the Taq polymerase and then 50 cycles of 10 sec at 95°C (denaturation) followed by 5 sec at 64°C (annealing) and by 10 sec at 72°C (extension).

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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