Fgd

used TaqMan probes directed against the Francisella tularensis tul4 and fopA genes. Detection limits for the BioSeeq were 200 and 300 fg, respectively; these are approximately 100 and 150 genome equivalents of F. tularensis. In comparison, the detection limit of the assay when performed on the ABI 7900 real-time thermal cycler instrument was 50 fg, approximately 25 genome equivalents. Assay specificity, determined by testing a panel of 27 bacterial species and strains, including other possible threat agents, was the same for both targets on both instruments. When DNA extracted from tissues of mice infected with the F. tularensis LVS strain via aerosol exposure was assayed on the ABI 7900 and the BioSeeq, results were comparable, indicating that the handheld instrument was successful in analyzing the DNA in reaction volumes half the size of those of the ABI 7900 (which uses a volume of 50 pl). Custom reagents for a variety of threat agents are expected to be available for the BioSeeq in the Winter of 2003/2004, and the device is projected to sell for $25,000.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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