Rnl

Fig. 2 Peak traces of multiplex capillary heteroduplex analysis (MCHA) depicting profiles for different categories of mutations. a) A 2-bp deletion in BBS2 x 4. i) Homozygous patient sample mixed with control DNA giving four species: a mutant homoduplex, a wildtype homoduplex, and two heteroduplexes; ii) control DNA homoduplex. b) A G! C substitution in BBS4 x 15. i) Patient sample; ii) patient sample mixed with control DNA; iii) control DNA homoduplex. c) BBS4 x 16 SNP. i) Heterozygous sample mixed with control DNA; ii) control DNA homoduplex. d) A T ! A substitution in BBS2 x 4. i) Patient sample mixed with control DNA; ii) control DNA homoduplex. (From Ref. [16].) (View this art in color at www.dekker.com.)

Single-base substitution UHG

Denature

Denature

Homoduplexes

Fig. 3 Schematic of the principle of the universal heteroduplex generator (UHG). The artificial mutation enhances the bubble effect of an existing mutation resulting in greater separation on gel electrophoresis.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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