Hla

Definition

Resolution

A2 A*02

A*0205/0208/0214

A*0201

A*0232N

DRB1*030101 and DRB1*030102 A*24020102La

Refers to the A2 antigen defined by monospecific/polyspecific antisera

Any of the 64 known A*02 alleles (A*0201-0264)

Any one of these three alleles; other A*02 alleles are excluded

Allele defined

Allele with a substitution in the coding sequence that leads to a stop codon (null allele) Two DRB1*0301 alleles that differ by a silent substitution in exon 2

A*2402 allele with low expression due to a mutation in intron 2 sequence

Low Low

Intermediate

High

High

High

High

"The first two digits refer to the serotype (A*24), the third and fourth digits define substitution(s) in the coding sequence (A*2402), the fifth and sixth digits describe synonymous substitution(s), and the seventh and eight digits refer to substitution(s) in intron or 5' or 3' sequences. N and L mark alleles with no or low surface expression, respectively.

end. A combination of two primers designed for each of the two polymorphic sequence motifs in cis allows the identification of an allele or a group of alleles that are characterized by these two motifs. The presence or absence of the two motifs in cis is usually detected by gel electrophoresis,1-11-1 but other detection methods have been developed.[12] The method is rapid and ideally suited for a small number of samples. The method can be applied for low-resolution typing, using primer combinations that detect all alleles within a given serotype, as well as for high-resolution typing. Depending on the HLA pheno-type, a combination of 300-400 different primer mixes is necessary to determine the HLA-A/B/C/DRB1/DRB3/ DRB4/DRB5/DQB1 alleles in a given individual.

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Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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