Info

PCR+sequencing[1314]

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PCR+RFLP[1S]

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Distinguish known types

No. of types

Find novel types throughput

'Media preserving the integrity and morphology of cells, e.g.. Sample Transport Medium (Digene), PreservCyte (Cytyc). b+=can be used on sample "as is" or on crude extract (proteinase K treatment/freeze-thaw/boil). c§=needs additional extraction/purification of DNA.

described. Table 1 gives an overview of the different techniques, sorted to typing resolution and throughput if applicable. In addition, the suitability for various clinical specimen types is indicated.

DESCRIPTIONS OF METHODS Nonamplification Methods

The best known technique in this category is the commercially available, FDA-approved Hybrid Capture 2 (HC2) method.[4] It detects 13 genital hr-HPV types by a mixture of full-length RNA probes; hence it is suitable only for group-specific detection and not for highresolution typing. Hybridization of one or more of the probes to HPV DNA present in heat-alkaline-denatured clinical samples is detected by peroxidase-labeled antibodies that recognize the RNA/DNA hybrid and visualized by chemiluminescence. The nonamplification nature of HC2 greatly decreases the risk of false positives by reaction product carryover. It is self-evident that the analytical sensitivity of this method is lower than that of PCR-based methods (Table 1). Some cross-reaction of the HC2 probes with HPV types not represented in the probe mix, including some nononcogenic HPVs, have been described.[5]

Polymerase Chain Reaction-Based Methods

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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