Fig. 6 Real-time PCR amplification with BEBO (0.2 mM) detection. Amplification was performed in a RotorGene with 470-nm excitation and 510-nm detection. Background is subtracted and the amplification curves are normalized. Samples are duplicates of a 1:1, 1:10, 1:100, 1:1000 dilution series of a 222-bp beta tubulin fragment and NTC. Inset: Standard curve (R = 0.99955, Slope - 3.8, Efficiency 0.83).

hybridization probes are examples of sequence-specific probes that are based on fluorescence resonance energy transfer (FRET) between two dyes tethered to an oligonucleotide. When these probes bind target DNA, the distance between the dyes changes as a result of either probe degradation or conformational change resulting in fluorescence enhancement. Common dyes in these probes are laser dyes with very high fluorescence quantum yields. An interesting alternative to the two-dye probes is the LightUp probe.[32] The LightUp probe has only one dye, which is a cyanine dye, whose fluorescence greatly increases when bound to DNA. Instead of using an oligonucleotide to identify the target sequence, the LightUp probe uses peptide nucleic acid (PNA), for which charged cyanine dyes have negligible affinity. The LightUp probes have been found to be excellent reporters in both real-time PCR and post-PCR applications.[33,34]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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