Info

Nucleotide

Enzyme

Allele

changes

activity

CYP2D6*1

None

Normal: wild type

CYP2D6*2

— 1584C>G

Normal

CYP2D6*2XN

Multiplication

Increased

CYP2D6*3

2549A>del

None

CYP2D6*4

1846G> A

None

CYP2D6*5

Deletion

None

CYP2D6*6

1707T>del

None

CYP2D6*7

2935A>C

None

CYP2D6*8

1758G>T

None

CYP2D6*35

31G> A

Normal

CYP2D6*41

— 1584C

Decreased

Source: Ref. [2].

705. In a second capillary, mutations *4, *6, and *8 were investigated by dual-color hybridization with LightCy-cler-Red 640 or 705. In addition, the LightCycler-Red-640-labeled probes of alleles *4 and *6 had different melting points.[15]

Another possibility to verify a deletion, a duplication and, in addition, a multiplication with the definite number of alleles is a quantitative PCR. Newly, a TaqMan real-time PCR was validated for the determination of the accurate gene copy number of CYP2D6.[16] Quantitative amplification data were normalized to albumin as a reference gene with FAM-labeled and VIC-labeled probes in a single-tube assay.[16] (For further information of the used probes, please see Table 2.)

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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