Normal: wild type
Source: Ref. .
705. In a second capillary, mutations *4, *6, and *8 were investigated by dual-color hybridization with LightCy-cler-Red 640 or 705. In addition, the LightCycler-Red-640-labeled probes of alleles *4 and *6 had different melting points.
Another possibility to verify a deletion, a duplication and, in addition, a multiplication with the definite number of alleles is a quantitative PCR. Newly, a TaqMan real-time PCR was validated for the determination of the accurate gene copy number of CYP2D6. Quantitative amplification data were normalized to albumin as a reference gene with FAM-labeled and VIC-labeled probes in a single-tube assay. (For further information of the used probes, please see Table 2.)
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.