Multiple PCR cycles

Fig. 3 PCR with functional primer(s). The procedure is as outlined in Fig. 1, but one or both primers contain an extra 5'-terminal functional segment. Here, it is shown only for primer B, indicated by a striped box and shown as tilted element for easier perception. During annealing of primer B, this box does not find a complementary target and sticks out, essentially without affecting PCR performance. After the third elongation step, this box is fully integrated in the specific PCR product and further amplified as shown before. (View this art in color at

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment