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100 (culture)

2001[191

aISR: intergenic spacer region: region between the (rrs)16S and (rrl) 23S rRNA genes.

bDescribed is the type of PCR, either single, nested, seminested or mutiplex. Also the type of detection of the PCR product is given, either by aISR: intergenic spacer region: region between the (rrs)16S and (rrl) 23S rRNA genes.

bDescribed is the type of PCR, either single, nested, seminested or mutiplex. Also the type of detection of the PCR product is given, either by

(colorimetric) probe detection or by gel analysis.

Vancomycin (3 mg/mL) should be added to reduce contamination by other microorganisms. Microscopically, the colonial structures have been described as ''schools of fish,'' ''railroad tracks'' and ''fingerprints.''[11] Serological assays were developed that detect either a past infection measuring IgG anti-H. ducreyi levels or active infection detecting IgA and IgM levels.[10,13] IgG-based assays are mostly used to perform epidemiological studies.[11] A rapid diagnostic test based on monoclonal antibodies to the hemoglobin receptor HgbA was highly specific, but the sensitivity was too low for on-site diagnosis.[14] The first H. ducreyi-specific PCR assays were developed in the mid-1990s (Table 1), and DNA amplification is nowadays considered the most sensitive diagnostic method. Multiplex PCR (M-PCR) was developed in which TP, HSV, and H. ducreyi are simultaneously detected.[9,10,20] M-PCR is highly efficient but not commercially available. Nevertheless, PCR should be used as reference standard in evaluations of novel diagnostic techniques.1-11-1

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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