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Fig. 2 Accuracy and reproductivity within run depend on different levels of proviral load. (View this art in color at www.dekker.com.)

systems developed is reported to be acceptable in terms of specificity, low detection limit (one copy per reaction), broad linear dynamic range, and reproducibility to be used both for qualitative detection and quantitative follow-up. Our real-time PCR system has also shown to be accurate and reproductive at different cell concentrations of 100-10"3 equivalent to about 100-0.1% infected cells/104 PBMCs in which 104 ATL cells harboring one proviral copy were diluted with normal lymphocytes, as shown in Fig. 2. The mean copies/104 PBMCs and coefficient values (CV) at each concentration were 8913±570, 655±32, 70±7, and 5±2, and 6.4%, 4.8%, 9.6%, and 51.8%, respectively. The between-day precision using the PBMCs from a seropositive healthy individual is reproducible with a CV of 15.9% and a mean of 189 copies. Now, Table 1 represents the practical data of the proviral load by real-time PCR in asymptomatic healthy carriers and patients with HAM/TSP, giving probably the approximate values.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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