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Fig. 2 Rapid analysis of sickle cell anemia polymorphisms. (A) The HyBeacon probe employed to genotype samples with respect to sickle cell disease was fully complementary to the sickle cell (HbS) allele, exhibiting single and double nucleotide mismatches when hybridized to normal b globin gene (HbA) and hemoglobin C (HbC), respectively. (B) HyBeacon probe hybridized to the fully complementary HbS allele possesses a Tm of approximately 58°C. Hybridization to the mismatched HbA and HbC alleles drops the probe Tm to 52°C and 48°C, respectively. Combinations of probe melt peaks allow determination of HbA/A, HbS/S, HbA/S, and HbA/C genotypes.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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