target sequences within the TK gene and/or the viral gene encoding DNA polymerase. Primers that may be used are summarized in Table 1. These techniques have been applied to the genetic characterization of resistant isolates detected with phenotypic tests.[28] The first step of the development of genotypic tests could be the optimization of PCR amplification to increase its sensitivity so that it could be performed directly on clinical samples. A specific PCR amplification of HSV TK gene, involved in 95% of resistance cases, followed, if positive, by PCR product sequencing could allow the detection of mutations associated with resistance to ACV. A complete database of the HSV mutations associated with ACV resistance or TK polymorphism is needed to interpret sequencing results. In this way, results could be available in 24 to 48 hr, allowing early change of antiviral treatment according to virus susceptibility.

As more and more data are available on TK mutations associated with resistance, the GeneChip technology, developed by Affimetrix (Santa Clara, CA) may be considered as a promising method for the detection of resistance. This method of genetic diversity analysis relies upon the hybridization of the nucleic acid target to a large set of oligonucleotides. This technology has already been applied to detect mutations into HIV and Mycobacterium species genome,[34,35] giving results in a few hours. Herpes simplex virus arrays could thus represent a rapid and automatable method to detect mutations into TK and DNA polymerase genes associated with resistance to antiviral drugs.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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