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Fig. 1 Schematic of the Invader® DNA assay. (View this art in color at www.dekker.com.)

Cleavage of the FRET oligo separates the donor fluorophore (F) from the quencher fluorophore (Q) to generate a fluorescent signal. Using two different 5'-flap sequences and their complementary FRET oligos, each labeled with different fluorophores, two different target sequences may be detected in a single well. In addition to producing signal, the FRET oligo provides a second level of signal amplification. In the DNA assay, the 5'-flap forms an unstable, invasive duplex with a FRET oligo. After the fluorophore has been released, the 5'-flap dissociates and is able to form an invasive structure with a new, uncleaved FRET oligo. Signal amplification results from the combined effect of having multiple flaps cleaved for each target molecule and from each of those released flaps driving the cleavage of multiple FRET oligos. Both reactions—cleavage of the primary probes and of the FRET probes—occur simultaneously at a single temperature near the melting temperature of the primary probe (Fig. 1).[3-5]

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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