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aHomology of primers to published virus sequences (GenBank); X/Y/Z score: X, number of published sequences overlapping the primer-binding site (the more the better); Y, sequences containing > 5 mismatches (the lesser the better); Z, sequences containing > 2 mismatches within 5 bases from the 3'-end of the primer or a mismatch at the ultimate 3'-base of the primer (the lesser the better). Abbreviations: g/ag, genomic/antigenomic primer; p, probe; SB, Southern blot; FAM, 6-carboxyfluorescein; TX, 6-carboxytetramethylrodamine. bEbola and Marburg sequences. cOnly Ebola-Reston sequences.

Only Ebola-Zaire sequences. eOnly Ebola-Zaire and -Sudan sequences.

aHomology of primers to published virus sequences (GenBank); X/Y/Z score: X, number of published sequences overlapping the primer-binding site (the more the better); Y, sequences containing > 5 mismatches (the lesser the better); Z, sequences containing > 2 mismatches within 5 bases from the 3'-end of the primer or a mismatch at the ultimate 3'-base of the primer (the lesser the better). Abbreviations: g/ag, genomic/antigenomic primer; p, probe; SB, Southern blot; FAM, 6-carboxyfluorescein; TX, 6-carboxytetramethylrodamine. bEbola and Marburg sequences. cOnly Ebola-Reston sequences.

Only Ebola-Zaire sequences. eOnly Ebola-Zaire and -Sudan sequences.

polymerase gene PCR.[9] The viral RNA concentration in a serum of a convalescent and an acute patient was 3 x 103-fold and 3 x 105-fold, respectively, above the detection limit of the real-time format of the polymerase gene PCR.[6] Taken the data on clinical and analytical sensitivity together, the polymerase gene PCR is a reliable tool to diagnose filovirus infections.

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