Fig. 2 Reverse phase protein microarray design. Protein microarrays may be designed as a single pad (A) or as sectors (B). The single pad design represents multiple samples, probed with a single antibody, on one microarray slide. Each sample is arrayed in duplicate in a 6-point dilution curve representing undiluted, 1:2, 1;4, 1:8, 1:16 dilutions, and a blank or negative control spot. Inclusion of a reference or control lysate permits process quality control within each slide. The single pad array design (A) allows multiple patient samples to be compared across arrays. Sector microarrays (B) represent one individual sample printed in multiple sectors and probed with a different antibody in each sector. The sample is prepared in a dilution curve in the same manner as for a single pad microarray. The removable reservoir effectively miniaturizes the assay, thus decreasing the volume of reagents for any particular assay. The sector array design allows analysis of multiple proteins for an individual patient on one microarray.

microarrays follows these general protocols: sample collection, sample preservation, preparation of frozen tissue sections, isolation of pure cell populations, protein extraction, microarray printing, and protein detection.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment