DNA extracts from ancient tissue remains often contain an unidentified component that inhibits polymerase activity. Inhibition can result from either reducing sugars[17] or an excess of coextracted microbacterial DNA,[18] the remains of porphirines,[19] degraded nucleic acids,[20-22] and soil components such as humic and fulvic acids, tannins, or iron ions (Fe2+),[23-25] or collagen type.[26] This inhibition may be overcome by the addition of bovine serum albumin and increased amounts of enzyme. Through the purification step by the microconcentrator device described above, the success rate of PCR is increased.

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