Introduction

To perform molecular biology studies based on the analysis of nucleic acids such as DNA and RNA, nucleic acid amplification technology is necessary because the gene content of almost all specimen is extremely low. Because these methods can be useful in the diagnosis of infections and genetic diseases, including predictive genetic and pharmacogenetic testing, diagnostic laboratories including hospital laboratories frequently use such technology as molecular diagnostic tools. In the medical field, amplification technology will play an important role in facilitating point-of-care testing to improve patient care. To widely apply such technology for practical use in the medical field, low cost and rapid performance are important. To achieve a cost-effective method, the apparatus being used must be simple. Moreover, the time from the start of a reaction until the result is obtained should be no more than 30-60 min. For example, methods amplifying genes under isothermal conditions have been developed (see other sections), but they require more than one enzyme and present difficulty in the selection of suitable reaction conditions.

To solve such problems, we have developed a new method of gene amplification. This method is named loop-mediated isothermal amplification (LAMP), and it involves gene amplification under isothermal conditions using only one enzyme with a high specificity, which is of particular importance for diagnostic techniques. Indeed, LAMP can be used for a direct detection of Mycobacterium tuberculosis in clinical diagnostic applications. However, compared to other methods [including polymerase chain reaction (PCR)], LAMP seems to be much less frequently used in laboratory diagnostics, because it was only developed recently or the principle and the primer design are a little complex (although the experimental procedure is simple).

In this section, we introduce the features and principles of LAMP, including the rapid method using a loop primer and single nucleotide polymorphism (SNP) typing.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment