Introduction

Genes of the major histocompatibility complex (MHC) encode human leukocyte antigen (HLA) molecules and have been implicated in the genetic background of many autoimmune and infectious diseases. Among the three classes of HLA molecules, class II genes, primarily the DR and DQ loci, are thought to be functionally significant in disease susceptibility; however, associations have been demonstrated with the class I and class II regions as well.[1] The human genome project has stimulated research on the relationship between specific HLA alleles and disease susceptibility, creating a need for high-throughput HLA typing. To demonstrate the methods of developing a microarray system for high-throughput HLA typing, DQA1, being the least complex molecule of class II HLA genes, was selected as a model. Understanding experimental conditions and accuracy of data analysis to distinguish allelic single-base changes will provide a foundation for the development of a DNA microarray to detect base changes in other HLA genes of interest.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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