Introduction

The qualitative and quantitative detection of RNA is clinically important. Reverse transcription of RNA followed by polymerase chain reaction (PCR) is a two-step reaction, which can be inconvenient; furthermore, it may be difficult to differentiate mRNA from contaminating genomic or proviral DNA. This review presents alternative RNA amplification strategies and describes the data published in studies that have used this methodological approach. Nucleic acid sequence-based amplification (NASBA) (bioMerieux, Boxtel, The Netherlands) and transcription-mediated amplification (TMA) (Gen-Probe Inc., San Diego, CA; Bayer Diagnostics Division, Tarrytown, NY; Chugai Diagnostics Science, Tokyo, Japan) amplify RNA under isothermal conditions. The constant temperature maintained throughout the amplification reaction allows each step of the reaction to proceed as soon as an amplification intermediate becomes available. Products of TMA and NASBA are single-stranded and thus can be applied to detection formats by using probe hybridization without any de-naturation step.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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