Introduction

The publication of the human genome sequence in 2001 and the completion of about 140 prokaryotic genomes affirm the success of worldwide genome sequencing projects. The availability of new reference sequence information of various organisms is continuously growing.[1]

Differential sequencing—the systematic comparison of genomic sequences with respect to reference sequences, also called resequencing—represents a central focus of current genome analysis. The exploration of single-nucleotide polymorphisms (SNPs) is one of the most prominent approaches to extract the medical and biological value of genome-sequencing data and to elucidate inter- and intraspecies genetic variations.[2] The analysis of SNPs in the human genome will have a significant impact on the identification of disease susceptibility genes and drug targets and will facilitate the development of new drugs and patient care strategies. Large-scale analysis, detection, and discovery of genetic variability and implementation of dense SNP maps are eminent and push technological development toward high-throughput, cost-efficient applications.

Differential sequencing of infectious agents for fast and reliable identification and typing is an important aspect in the field of molecular diagnostics and epidemiology, including outbreak tracking and classification of pathogens. Fast and highly accurate tools for identification, monitoring, and treatment control are in demand. This article describes the use of mass spectrometry to rapidly identify and localize variable genomic regions based on reference sequences, thus facilitating high-throughput differential sequencing with a gamut of applications.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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