Polymerase chain reaction (PCR) is a well-established, powerful diagnostic tool that provides rapid, highly sensitive, and specific DNA or RNA detection. The demand for equally rapid and sensitive methods to achieve detection and semiquantitative information on PCR products has brought about the coupling of PCR to enzyme-linked immunosorbent assay (ELISA). These assays involve hybridization of amplicon with specific oligonucleotide probes (thus enhancing the specificity of the method) and detection of the hybridization product with suitable enzyme-labeled antibodies. A further advance of the technique involves the use of chemilumi-nescence as detection system, yielding PCR chemilumi-nescent enzyme immunoassay (PCR-CLEIA). This further improves the rapidity and sensitivity of the technique and lets us envisage future developments toward miniaturized and automated high-throughput methods.
In this paper, the principle of the method, applications, and future trends of PCR-CLEIA are presented.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.