Further development and improvement of molecular diagnosis depends on the development of robust, automatable, and cost-efficient technologies. In addition to conventional methods, a variety of homogeneous amplification assays that are based on fluorescence are available and facilitate detection of amplicons and characterization of mutations, single nucleotide polymorphisms (SNPs), and other sequence variations in DNA. Especially, SNPs have recently gained great attention, being markers for susceptibility to disease or response to therapeutic drugs.

To serve the increasing request for the simultaneous determination of larger numbers of parameters, DNA arrays (DNA chips) of different densities are available that allow high throughput and multiplex sequence and gene expression analysis. They require dedicated automation that may not be available in routine laboratories and their capabilities exceed by far what is currently required for diagnostic purposes. These conventional DNA and oligo-nucleotide arrays consist of a variable number of spatially arranged spots with nucleic acids on solid supports. The production of such arrays in small numbers for specific questions may be time consuming and costly. In addition, high-density arrays may not be necessary for many diagnostic purposes.

An interesting alternative to DNA chips is bead-based technology. The main advantage of this technology is that different species of beads are coated with the desired oligonucleotides prior to mixing the distinct populations together. A so-called suspension array for multiple analyses can be generated and modified according to the individual needs. Such suspension arrays can be supplemented with additional bead populations any time.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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