Introduction

A reliable approach to quantitative PCR is defined as competitive PCR (c-PCR) and it is based on the addition to the sample of a competitor target (DNA or RNA), acting as an internal standard, which has the same primer recognition sites as the target gene and, consequently, the same behavior during PCR cycling. The PCR amplicons can be then separated by conventional procedures and quantitation is achieved by comparing the signal of the specific target with that obtained from the known concentration of the internal standard (competitor template). This strategy seems to guarantee the independence of the results from any predictable or unpredictable variables that can affect the rate of amplification because the two species are amplified at the same rate and their ratio remains constant during PCR cycling. With this procedure, the parallelism between target and competitor is maintained even after reaching the plateau phase, with an increase in the assay sensitivity.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment