Introduction

With the excitement and knowledge gained from the completion of the Human Genome Project, our understanding of underlying genetic determinants in monogenic and complex diseases promises to exponentially increase over the coming years. These developments should also prove to be a boon for the field of molecular diagnostics and improve our ability to diagnose and treat genetic diseases. A number of technologies, which are routinely applied toward mutation detection in the clinical laboratory setting including single-strand conformation polymorphism, denaturing gradient gel electrophoresis, denaturing high-performance liquid chromatography, and direct sequence analysis, exist today. The sensitivity of mutation detection in all of these methods is hampered by the presence of the wild-type allele, which can obscure the mutant, making it difficult to differentiate between the two. Monosomal cell hybrids can be created through the disruption of cell membranes between two cells in contact, which leads to the fusion of the membranes and the formation of a hybrid cell. When hybrids are formed between human and rodent cells, the resulting progeny will retain a random assortment of human chromosomes, which can be specifically selected for. By utilizing hybrids that are monosomal for any specific human chromosome in diagnostic testing, the sensitivity of these existing techniques can be improved by allowing each chromosome to be analyzed independently.

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Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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