Introduction

Real-time PCR methods are of great importance to a multitude of diagnostic sectors, such as health care, forensics, food analysis, and defense, which require rapid genetic analysis. Traditional methods of sequence analysis are time consuming and need to be performed in specialized facilities, requiring multiple steps and techniques to achieve target amplification and detection. A range of fluorescent probe technologies, such as TaqMan, Scorpions, molecular beacons, and hybridization probes, has been developed to combine the target amplification, detection, and analysis processes required to type samples. The combination of amplification and detection in homogeneous real-time PCR reduces the duration of tests, often to less than 30 min, and decreases the possibility of cross-contamination. Such rapid analyses may be performed at the point-of-care (e.g., at hospitals and doctors surgeries) and in-the-field (e.g., on farms and battlefields) by individuals provided with minimal training.

HyBeacon™ probes provide a homogeneous method of ultrarapid sequence analysis that allows samples to be genotyped in less than 20 min. These fluorescent probes are capable of reliably detecting specific DNA targets and discriminating closely related sequences, including those containing single nucleotide polymorphisms (SNPs). As such, HyBeacon probes may be employed in a large number of applications including genetic diagnostics, disease predisposition, pharmacogenetics, and pathogen detection. HyBeacons exhibit a simplistic mode of action, possess few design constraints, yield easily interpreted melt peak data, and can simultaneously analyze multiple target sequences with a single probe. HyBeacon assays have been demonstrated to function efficiently directly from samples, such as saliva, without prior purification of nucleic acids, making the probe technology suitable for point-of-care diagnostics.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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