Introduction

Real-time monitoring of the polymerase chain reaction (PCR) product by fluorescent probes has recently enabled the homogeneous detection of pathogen deoxyribonucleic acid (DNA) or ribonucleic acid (RNA0) in a closed-tube format. The reduced risk of carry-over contamination and the superior sensitivity of PCR-based assays have made real-time PCR the method of choice in many clinical settings. The LightUp® Probe is a novel of fluorescent probe where the fluorescent reporter dye is coupled to an oligomer of the uncharged DNA analog peptide nucleic acid (PNA). Peptide nucleic acid provides strong, but still specific, binding and serves as the sequence-recognizing element of the probe. The fluorophore is an asymmetric cyanine dye that fluoresces when the probe binds its target DNA, but is virtually nonfluorescent when the probe is free in solution.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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