Introduction

Genomic DNA is used in a wide range of applications in molecular biology including disease diagnosis, food microbiology, and environmental microbiology. However, DNA analysis in many aspects of medicine and biology rely on the extraction of DNA from complex samples or environments. These may possess numerous contaminants depending on the sample type. In medicine, the extraction of human genomic DNA from tissue samples may be contaminated with commensal bacterial flora. In environmental microbiology, the analysis of water or soil samples may be contaminated by bacterial DNA, plant DNA, and minerals. Interestingly, the analysis of DNA has led to the revised classification of some soil bacteria. Even the complex environment of human blood hampers the detection of infection because of the presence of red blood cells, white blood cells, transient contaminant bacteria, and numerous components of the immune system. Therefore the technique used for DNA extraction is important, as are the steps performed during the process such as cell lysis, DNA recovery, and DNA purification. In medical microbiology, there is a growing interest in molecular methods for the laboratory confirmation of infection and advances in molecular biology have promoted the routine use of techniques such as PCR and DNA sequencing. However, difficulties exist with the extraction of genomic DNA from bodily fluids. Sensitive DNA extraction methods are required but the DNA extracted must be of sufficient quality to be used for the improved diagnosis and surveillance of microbial diseases. In all of the areas above, traditional phenol-chloroform extraction is not usually practical because of the time and safety constraints of the method. Here DNA extraction systems are compared in terms of ease of use, sensitivity, and their ability for automation. An emphasis is placed on their use in clinical microbiology, particularly in relation to the use of whole blood for the diagnosis of microbial infections.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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