This review summarizes present knowledge on the clinical implications of real-time polymerase chain reaction (PCR), a novel fluorescence-based method for the quantitation of gene expression and gene copy number variations, as well as gene amplification and locus haploinsufficiencies in neuroblastomas. Particular emphasis is placed on precision and accuracy and the comparison of TaqMan® real-time PCR with other techniques and devices for quantitative real-time PCR, and more traditional methods for the quantitation of gene expression such as competitive quantitative PCR. Finally, applications with potential clinical implications, such as the detection of minimal residual disease (MRD) or the assessment of tumor malignancy, are discussed.
Was this article helpful?
The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.