Introduction

The rolling-circle amplification (RCA) reaction is an isothermal in vitro method for the hybridization-triggered enzymatic synthesis of hundreds to billions of linear copies of small, single-stranded, circular DNA probes. Thus, obtained long repeats of DNA sequences may serve as a signal amplifier for ultrasensitive detection of specific nucleic acids and other biologically important molecules in diagnostic genomics and proteomics. Depending on the goal and/or target molecule to be detected, RCA is performed using a variety of protocols and testing formats with either linear or exponentially branching kinetics. Because of their robustness and simplicity, the RCA-based assays hold a distinct position in the area of molecular diagnostics among other single-temperature amplification techniques.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment