Introduction

The polymerase chain reaction (PCR) has been adapted for a myriad of applications involving the amplification of nucleic acid sequences. This technology often involves a specific detection methodology to analyze the products of a reaction. The advent of real-time detection systems, which detect amplified products concurrently with the amplification process of the reaction, has enabled the creation of a system that can provide accurate, quantitative results without cumbersome postamplification steps. Multiplex real-time PCR technology is simply a variation of the standard reaction methodology in which there are multiple amplification reactions that take place in a single reaction vessel. The ability to combine reactions together provides a number of benefits. Not only does it allow for multiple target detections, which saves time, effort, and money, but it also provides a means for enhanced experimentation. For instance, with the ability to discriminate different targets in a reaction, comparative or relative quantitation of targets can more accurately be achieved, and positive controls can be coamplified with targets of interest to monitor the efficiency of each individual reaction. There are a number of different chemistries available that allow for multiplex real-time amplification and discrimination of targets. Each chemistry and each multiplexing strategy has its advantages as well as its own technical challenges. However, if these challenges can be overcome, this ''simple'' variation of a standard PCR reaction turns into an extremely powerful tool for clinical diagnostics.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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