Introduction

The capacity to separate nucleic acids and proteins by their molecular weight and identify fractions of interest by the hybridization of specific labeled probes is known as blotting. It was originally developed for the analysis of gel-fractionated DNA and was termed the Southern blot. Northern blotting was soon to follow and permitted the investigation of RNA templates by using an identical procedure. Since then, Western blotting was designed to analyze proteins and a procedure known as Dot/Slot blotting was developed to detect and quantify any nucleic acid sequence without the necessity of processing [restriction fragment length polymorphism (RFLP) and/ or separation by electrophoresis] the nucleic acids.

The development of blotting techniques has helped revolutionize molecular biology. One of the benefits of detecting nucleic acids using a blot/probe system, rather than ethidium bromide staining, is that it provides an enhanced sensitivity and confirms the identity of the polymerase chain reaction (PCR) amplicon or target sequence. Initially, targets were visualized by hybridization with a radiolabeled (32P) probe, but because of apprehensions over user safety/exposure and the difficulty with disposal of radioisotopes, a number of nonisotopic alternatives are now available allowing colorimetric, chemiluminescent, or fluorescent detection.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment