Introduction

The polymerase chain reaction (PCR) is a common tool of molecular biology and is utilized in the field of medical diagnostics to identify genetic disorders and the causative agents of human infections. PCR is also used for typing microorganisms, thus playing an important role in both epidemiology and phylogenetics. The PCR method is a starting point that when used together with cloning is influential in the sequencing of novel genes and genomes.

When compared to cloning techniques, PCR is both more economical and straightforward to perform; however, it is limited by the fidelity and size (<5 kb) of the amplicon generated. Despite various publications1-1-3-1 reporting longer PCR amplifications (>10 kb), only low yields of amplicon are produced, thus limiting further applications.1-4-1

Long-distance/range PCR (LD-PCR) has been developed to overcome these restrictions and allows amplification of up to 40 kb.[4-7] This methodology has extensive applications in the field of molecular biology and these will be discussed later.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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