Introduction

The protein truncation test (PTT) is a mutation detection technique that specifically detects mutations leading to the premature termination of protein translation. An increasing number of genes implicated in disease processes (primarily cancers) have been identified where the majority of the mutations result in premature termination of translation leading to an incomplete and nonfunctional protein product. These include the APC gene,[1'2] BRCA1 and 2,[3] PKD1,[4] NF1,[5] TSC1 and 2,[6] and DMD.[7] Protein truncation test was first reported in 1993[1,8] and has been used mainly in clinical research settings (http://www.genetest.org). However, until recently the PTT had not significantly advanced beyond its original form and has not gained wider acceptance because of several limitations that include 1) slow readout because of the use of electrophoresis followed by radioactive detection; 2) errors in the visual detection of mobility shifts on a gel (which depend on the level of the training of the technician); 3) safety issues involved with the use of radioactivity; and 4) the difficulty in automating SDS-PAGE. We describe here recent advances in the PTT including the introduction of an ELISA-based PTT (ELISA-PTT), and how these advances might lead to wider acceptance of this promising yet underutilized technique.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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