Introduction

NEBcutter is a computer program offering services to research laboratories that employ nucleic acid technologies. The software provides bioinformatics tools for experiments where DNA molecules are manipulated with restriction enzymes. Type II restriction enzymes recognize specific four- to eight-nucleotide-long patterns in DNA, and introduce cuts into the strands at predictable positions.[1'2] The specificity and precision of cleavage make these enzymes useful in a wide array of applications. Gene cloning, detection of point mutations, probing of repeat sequences, genome mapping, and characterization of genetic variations1-3-5-1 are a few of these. The NEBcutter program supports such applications by providing routines for the systematic analysis of restriction site distributions within defined DNA sequences. The service is accessible over the web, through a link on http:// www.neb.com.

The discovery of restriction enzymes and the accumulation of significant amounts of sequenced DNA prompted the development of the first restriction digest analysis computer programs.[6,7] Since then, hundreds of new restriction enzymes have been characterized. Today, the authoritative restriction enzyme database, REBASE,[8] lists over 3500 type II entries, with almost 250 different specificities. The amount of sequenced DNA has expanded equally rapidly. The GenBank[9] collection of DNA sequences now contains over 30 billion bases, including complete genome sequences of dozens of species (human, mouse, fruitfly, yeast, bacteria, and so on). Substantial changes in computer power and computing environments occurred during this time as well. Advanced algo-rithms,[10] network access, linked databases, and graphic presentations have become standard. In this new environment, NEBcutter provides tools for state-of-the-art restriction enzyme digest analysis.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook


Post a comment