Introduction

The introduction of real-time PCR in routine diagnostic laboratories has markedly increased the convenience and speed of PCR-based diagnostic assays. As product detection and identification in real-time PCR is performed within closed PCR vessels, the necessity for cumbersome post-PCR analysis steps is omitted. When real-time PCR is carried out in glass capillaries, e.g., on the LightCycler instrument, the PCR cycling speed is markedly increased to approximately 30 sec per cycle only. This rapid-cycle real-time PCR permits product detection by the utilization of various formats of fluorescence dye technologies such as fluorescence resonance energy transfer (FRET) hybridization probes, TaqMan probes, molecular beacons, or SYBR Green I. With this technique numerous protocols have been developed for diagnostic purposes. The sensitivity of rapid-cycle real-time PCR has been demonstrated to the extent of detecting a single copy of DNA per reaction vessel. In order to achieve this high technical sensitivity, the amplification reaction is required to run highly efficiently. However, this may not always be obtained in diagnostic applications. Sequence constraints may prevent ideal primer and probe design, and the complexity of nucleic acids from a clinical sample may preclude ideal amplification conditions.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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