IPCR of a Linearized Construct

An alternative procedure for PCR amplification of an unknown target requires that the circular construct produced as described in ''IPCR within a Circular Construct'' should be linearized by using a restriction enzyme located in the known sequence between the 5' regions of the PCR primers. This additional step has been shown to increase the efficiency of the PCR amplification reaction up to 100-fold.[4] In theory, covalently closed circular double-stranded DNA is a poor substrate for PCR with reannealing of the DNA strands during the extension step of PCR. However, linearization or nicking of such a construct will reduce this and make it a more ideal template for amplification (Fig. 2).

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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