Accurate interpretation of signaling pathways is dependent on the proportion of cells contributing to the total signal for a given cell population. Laser capture microdissection (LCM) is an established technique for procurement of pure cell populations from heterogeneous tissue sections.[29,30] Microdissection permits procurement of cells from any designated area of a tissue biopsy, such that cells representing normal, diseased, or stromal tissue may be obtained in relation to their microenvironment. Proteins from neighboring, contaminating cells will not be microdissected, thus limiting their contribution to the overall repertoire of proteins in the population of cells to be studied. Protein microarray construction may be performed with as few as 5000 microdissected cells/15 pL of extraction buffer.
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The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.