Locus Specific Pcrbased Rflp Technical Description

In recent years, a number of PCR-based strategies have been developed for strain discrimination of microbial pathogens. In PCR gene RFLP typing, a target sequence, 1 to 2 kb long and known to show polymorphism among strains of species of interest, is amplified at high stringency. The amplified product is cut with restriction endonucleases and isolates are compared by RFLP pattern on an agarose gel after staining with ethidium bromide avoiding the need for Southern blot.

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Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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