Materials And Methods Patient Selection

A total of 78 cases tested for the PML/RARA rearrangement by FISH were retested with a RARA break-apart probe. Of these 78 cases, 27 were examined for the PML/RARA rearrangement by FISH to rule out M3 variant (M3v) at initial diagnosis, 16 cases were typical APL cases, which were positive for the PML/ RARA rearrangement by RT-PCR at the time of initial diagnosis, and 21 cases were APL cases for follow-up after induction or consolidation therapy. Fourteen cases with hematological malignancies (non-APL) during the follow-up were included to determine whether there were any cutoff value differences when patients are under chemotherapy vs. the normal controls. To establish the reference range, PML/RARA and RARA FISH were conducted on specimens obtained from bone marrow examinations conducted in 20 normal controls without hematological malignancies (donors for bone marrow transplantation) and from the peripheral blood of 30 healthy persons. We scored the signals in 200 nuclei in each of the samples. Bone marrow mononuclear cells stored in methanol at — 20°C were used for interphase FISH staining.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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