Melt Peak Analysis

Because the amount of fluorescence emitted from HyBeacons is determined by the hybridization state of the probe, the nature of target sequences may be determined by melting curve analysis (Fig. 1). At low temperatures, probes are hybridized to target sequences and emit elevated levels of fluorescence. As temperature increases, the level of fluorescence emission reduces as probes dissociate (melt) from their target sequences. The melting temperature (Tm) of the probe occurs when 50% is hybridized to target sequence and 50% is single-stranded. Probes hybridized to fully complementary target sequences are considerably more stable than those bound to targets containing regions of mismatch (Fig. 1). Therefore, closely related target sequences, differing by as little as a single nucleotide may be reliably differentiated on the basis of Tm. Melt peaks are highly specific and reproducible such that probe Tms may be employed directly to determine the nature of target sequences.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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