Melting Temperature Prediction

The nearest-neighbor melting temperature thermodynamic ic model1 ] is represented by AH

+ 16.6log10[xsalt]

where

GC AT

sym sym and where AH, and AS, are the enthalpy and entropy terms associated with the n — 1 pairs of neighboring nucleotides contained in the sequence of interest. R is the universal gas constant (1.987 cal/mol K). Ct is the total molar concentration of single strands when oligonucleotides are self-complementary or it is equal to 1/4 of this concentration in the case of non-self-complementary m sequences. The model assumes an equal concentration of the complementary strands.

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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