Methods And Results

The basic methodology of generating a gene catalogue by EST sequencing is outlined in Fig. 1. The reader may refer to Refs. 1-3 for details on the EST production process and for the importance of having EST sequence information in the framework of a genome project.

The EST annotation pipeline of ESTAnnotator[4'5] (Fig. 2) can be divided into the following subsections: the preparation of quality-checked input EST sequences (shown in the upper part), the initial analysis of EST reads by database searches (shown at the left lower part), and the clustering of overlapping EST sequences as well as subsequent contig analysis (shown in the right lower part).

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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