Microarray Formats

Forward phase microarray

There are currently two approaches to producing arrays capable of generating this type of network information. The first format is the forward phase array, in which a labeled bait molecule, typically an antibody, is immobilized on the substratum.[1-6] Each spot represents only one type of bait molecule or antibody. The array is incubated with only one test sample containing several different analytes of interest. The captured analytes are detected with a second tagged molecule that recognized the captured analyte or by labeling the analyte directly.

Reverse phase microarray

Reverse phase microarrays consist of immobilized analyte molecules, with multiple analytes in each spot (Fig. 1). Each spot represents an individual test sample, allowing an array to be comprised of multiple, different samples, such as clinical biopsy samples. The reverse phase array is probed with a single detection molecule, and a single analyte is measured for each spot on the array, across multiple samples. This format allows multiple samples to be analyzed under the same experimental conditions for any given analyte. The arrays are probed separately with two different classes of antibodies to specifically detect the total and phosphorylated forms of the protein of interest. Comparison of the proportion of phosphorylated proteins across patients on the same array provides insights into the cellular signaling network for individual patients. Each slide microarray is probed with a different antibody, generating a set of microarray slides for each set of probe antibodies. Construction of reverse phase

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

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