The true microarray formats are basically sophisticated ASO dot blots on a smaller scale within an electronically controlled hybridization instrument. The current Nano-Chip from Nanogen consists of 33 mutations, including the ACMG-recommended panel. Each NanoChip consists of 99 test sites (electrodes) attached to platinum wire connections. The negatively charged DNA is electronically guided to a test site where biotinylated samples bind to streptavidin in the site. Following denaturation, fluorescent probes are hybridized to the array and signal is detected after stringent washing procedures. Advantages of this technology include the flexibility of reflex testing, the adaptability of chip design to customization and addition/deletion of mutations in the panel, and the self-contained nature of the constituents. Other manufactured microarrays for CFTR mutations in slightly earlier stages of development include those from Motorola Life Sciences, BioArray Solutions, GeneOhm, and Autoge-nomics. Depending on the density of the array on the manufactured chip, theoretically there would be no limit to the number of CFTR mutations that could be addressed, going well beyond the current recommended panel of 25. Indeed, by using an ultra-high-density array of the type

Getting Started With Dumbbells

Getting Started With Dumbbells

The use of dumbbells gives you a much more comprehensive strengthening effect because the workout engages your stabilizer muscles, in addition to the muscle you may be pin-pointing. Without all of the belts and artificial stabilizers of a machine, you also engage your core muscles, which are your body's natural stabilizers.

Get My Free Ebook

Post a comment